fapR [2017-8-11 16:0:3]

repressor of fatty acid synthetic genes

Locus: BSU15880

Isoelectric point: 5.39

Molecular weight: 21.26 kDa

Protein length: 188 aa

Gene length: 564 bp

Function: regulation of fatty acid biosynthesis

Product: transcriptional repressor

Essential: no

Synonyms: ylpC

Genomic Context

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Categories containing this gene/protein

• Metabolism → Lipid metabolism → Biosynthesis of lipids → Biosynthesis of fatty acids
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other

Gene

Coordinates: 1,661,967 → 1,662,533

The protein

Catalyzed reaction/ biological activity

• FapR regulates the expression of at least 10 genes (the fap regulon). It autoregulates its own expression. Malonyl-CoA, a precursor of fatty acid biosynthesis, binds to FapR changing its conformation to a non-DNA binding state. Hence, conditions that cause malonyl-CoA accumulation, like fatty acid biosynthesis inhibition, derepress the fap regulon.

Protein family

• fapR family (according to Swiss-Prot)

Effectors of protein activity

• malonyl-CoA and malonyl-ACP]] act as the molecular inducer of the FapR regulon PubMed

Structure

• 2F3X (complex with effector), 2F41 PubMed

Expression and Regulation

Operons

• Genes: fapR-plsX-fabD-fabG-acpA
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • FapR: repression, PubMed, in FapR regulon
  • ComA: activation, (ComA) PubMed, in ComA regulon

  Regulation

  • FapR: repressed in the absence of malonyl-CoA or malonyl-ACP (FapR) PubMed
  • expression of the operon is strongly reduced during biofilm formation PubMed

Biological materials

Mutant

• MGNA-B138 (ylpC::erm), available at the NBRP B. subtilis, Japan

References

Reviews

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The FapR regulon

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Original publications

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